Donald Duck sings this song in "The Volunteer Worker", and "The Riveter".
The song appears, with altered lyrics, at the finale of Walt Disney's Enchanted Tiki Room at Disneyland and Walt Disney World's Magic Kingdom, and is also used at the Seven Dwarfs Mine Train attraction.
In 1955, Jack Pleis recorded it for his album, Music from Disneyland.
The song was also featured in the 1979 stage adaption of the 1937 animated musical film.
In the 1988 Disney animated film Oliver & Company, Tito sings "Heigh-Ho, Heigh-Ho, it's off to work we go" when he is rescuing Jenny.
The group Mannheim Steamroller covered the song on their 1999 album, Mannheim Steamroller Meets the Mouse.
Los Lobos recorded a Spanish-language cover of this song for their 2009 album Los Lobos Go Disney.
On the 2011 album V-Rock Disney, which features visual kei artists covering Disney songs, Cascade covered this song.
On 2012 album Disney – Koe no Oujisama Vol.2, which features various Japanese voice actors covering Disney songs, this song was covered by Hiroshi Kamiya.
One of the most famous scenes in the 1984 movie Gremlins has the Gremlins watching the "Heigh-Ho" scene in a theater in Kingston Falls, and singing along.
Tom Waits recorded a version of "Heigh-Ho" for the 1988 A&M record Stay Awake: Various Interpretations of Music from Vintage Disney Films. The song was included on the "Bastards" Disc from his 2006 album Orphans. Bruce and Bongo also created a remix based on this song in 1986 "Hi Ho (Heigh Ho – Whistle While You Work)", reaching No1 in the Austrian charts. The song was also used in a 2010 Hershey's Kisses commercials, Biobest vitality commercials in Canada, and the Levi Strauss & Co. "Go Forth" ad campaign centered on Braddock, Pennsylvania.
In the Discworld novel series, the song – and several bawdy variations – is only obliquely referred to as the Heigh-Ho Song. A much enjoyed (although self-admitted very stereotypical) song, it's also one of the few popular dwarf songs not about gold.
The scene was also featured as a background during the closing credits in the 1996 fantasy-horror TV show Liham ng Gabi.
A version of the song by Bunny Berigan and his Orchestra is featured in a recent Levi's commercial entitled "We Are All Workers".
In the last scene of Scared Shrekless, the song can be heard as the dwarfs silhouettes are seen on a rock as the dwarfs walk along shortly before they are egged by Shrek and the gang.
Brian Wilson covered it in a medley on his album In the Key of Disney, which was released on October 25, 2011.
Polskie Radio Program III (Trójka) uses "Heigh-Ho" as the jingle of its Economical Informator.
The song is also briefly referenced in Snow White and the Huntsman when the dwarf Beith (Ian McShane) speaks the song's lyric "Hi-ho lads, it's off to work!" Another dwarf threatens to "smash his face in" if he starts whistling.
The entire song would later be used in Noah Byrd's black-and-white musical short of the same name, Heigh-Ho Mine. Oswald the Lucky Rabbit and the 1930s versions of Mickey Mouse and Horace Horsecollar make cameos. Tom Kenny, Dee Bradley Baker, and Richard Horvitz does every voice in the Cartoon except for the usage of audio of the Dwarfs from the soundtrack of Snow White and the Seven Dwarfs.
In the Saved by the Bell episode "Snow White and the Seven Dorks", the class is discussing ways to alter the story, Zack Morris suggests to "make the dwarves tall blonde chicks and put them in bikinis", to which A.C. Slater replies "I'm all for that, heigh ho heigh ho"!
In Boy Meets World, Eric comments that the family is up early, to which Alan replies "Someone was in the shower singing hi-ho, hi ho" and then Eric finishes his sentence with "It's off to work we go".
In Final Fantasy IV, when the player visits an underground town populated with NPC dwarves, most of them incorporate "Hi-ho" into their sentences when spoken to, a simplified form of Heigh-Ho.
In February 2017, Delta Air Lines released an add titled 4 am that feature this song.